Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231.
- Author:
Ya-lin XIE
1
;
Ji-zhen LIANG
;
Fei-ye LIU
;
Jun-yi ZHANG
;
Jian-ming XIE
;
Rong-cheng LUO
;
Rong LI
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; genetics; pathology; Cell Line, Tumor; Cell Proliferation; Female; Gene Transfer Techniques; Humans; Protein Tyrosine Phosphatase, Non-Receptor Type 6; genetics; metabolism
- From: Journal of Southern Medical University 2010;30(5):1024-1027
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.
METHODSThe eukaryotic expression vector pEGFP-C3-SHP-1 was constructed and transfected into breast cancer cell line MDA-MB-231 via Lipofectamine 2000, and the positive clones were selected using G418. SHP-1 expression in MDA-MB-231 cells was detected with immunocytochemistry and Western blotting, and the cell growth curve was observed using MTT assay.
RESULTSSHP-1 was highly expressed in transfected MDA-MB-231 cells, whose proliferation was significantly inhibited (P<0.05).
CONCLUSIONSHP-1 gene transfer into MDA-MB-231 cells results in inhibition of the cell proliferation.
