Effect of imatinib at different concentrations on rat C6 glioma cell apoptosis and cell cycle.
- Author:
Ling YANG
1
;
Zhong-yuan XU
;
Xiao-hua CHEN
;
Ke-wan WANG
;
Guo-feng LI
;
Zhi-liang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Benzamides; Cell Cycle; drug effects; Cell Line, Tumor; Dose-Response Relationship, Drug; Glioma; pathology; Imatinib Mesylate; Piperazines; pharmacology; Pyrimidines; pharmacology; Rats
- From: Journal of Southern Medical University 2010;30(5):1089-1091
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of imatinib on rat C6 glioma cell apoptosis and cell cycle.
METHODSMTT assay was used to determine the OD value of C6 glioma cells following treatment with imatinib at different concentrations (0.156, 10 and 15 micromo/L) for 24, 48 and 72 h. The cell apoptosis was assayed by Hochest/PI staining and the cell cycle changes were analyzed by flow cytometry.
RESULTSImatinib treatment resulted in increased number of apoptotic cells in a time- and dose-dependent manner. A 72-h treatment of the cells with imatinib at 10 and 15 micromo/L caused increased cell percentage in G(0)/G(1) phase to (68.53-/+0.83)% and (70.41-/+0.62)%, (P<0.01), decreased the percentage of G(2) phase cells to (14.48-/+0.12)% and (13.84-/+2.86)% (P<0.01), and decreased the percentage of S phase cells to (16.98-/+0.72)% and (15.78-/+2.28)%, respectively (P<0.01).
CONCLUSIONImatinib can induce apoptosis and affect the distribution of the cell cycle of C6 cells in vitro.
