Hath1 gene transfer inhibits the proliferation of colonic cancer cells in vitro.
- Author:
Dai-hua ZHU
1
;
Jian-ping GONG
;
Ke REN
;
Jian-ming SUN
;
Si-dong WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basic Helix-Loop-Helix Transcription Factors; genetics; metabolism; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; pathology; Cyclin D1; genetics; metabolism; Gene Transfer Techniques; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Mucin-2; genetics; metabolism; Proliferating Cell Nuclear Antigen; genetics; metabolism; RNA, Messenger; genetics
- From: Journal of Southern Medical University 2010;30(5):1005-1008
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Hath1 gene transfer on the proliferation of colonic cancer cells in vitro.
METHODSThe recombinant plasmid pcDNA3.1(+)-Hath1 was transfected into HT29 colonic cancer cells, and 3 positive cell clones were randomly selected to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time RT-PCR and Western blotting. The proliferation of the transfected HT29 cells was observed by means of colony formation assay and xenograft growth in nude mice.
RESULTSHath1 significantly down-regulated of cyclin D1 and up-regulate of p27 expressions and inhibited the proliferation of HT29 cells.
CONCLUSIONHath1 gene may be an anti-oncogene in colon carcinogenesis.
