Changes of aquaporin-1 expression in rat myocardium after severe burn.
- Author:
De-quan XIAO
1
;
Jia-han WANG
;
Zhi-qing LI
;
Zhao-hui YI
;
Li Xiao-zi XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aquaporin 1; genetics; metabolism; Burns; metabolism; pathology; Edema; metabolism; Female; Male; Myocardium; metabolism; pathology; Random Allocation; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2010;30(5):1111-1113
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in the myocardial expression of aquaporin-1 (AQP1) protein and its association with myocardial edema in rats with severe burns.
METHODSForty-eight healthy adult Wistar rats were randomly divided into normal control group (n=6) and burn injury group with third degree burn of 30% total body surface area, and the latter group was further divided into 2, 4, 8, 12, 24, 48 and 72 h groups. The changes of myocardial water content were investigated by dry-wet weight methods. Enzyme-linked immunosorbent assay was used to detect the changes in AQP1 expression at different time points after sever burns.
RESULTSThe myocardial water content and AQP1 expression increased significantly 2 h after the burn injury, reaching the peak levels at 12 h and remaining higher than the normal level at 48 h. A significant positive correlation was found between myocardial water content and AQP1 expression in the rats (r=0.868, P<0.01).
CONCLUSIONThe severity of myocardial edema after severe burn is correlated to the expression level of AQPl protein, suggesting the important role of AQPl protein in pathological progression of myocardial edema.