Mechanisms of foam cell formation in smooth muscle cell derived from bone marrow stem cells.
- Author:
Peng-ke YAN
1
;
Shi-huang LI
;
Cai-wen DUANG
;
Zheng-Rong MEI
;
Xiang-ping TAN
;
Tu-bing SI
;
Guo-hong XIAO
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters; metabolism; Animals; Bone Marrow Cells; cytology; Caveolin 1; metabolism; Cell Differentiation; Cells, Cultured; Female; Foam Cells; cytology; Lipoproteins, LDL; pharmacology; Male; Mesenchymal Stromal Cells; cytology; Muscle, Smooth, Vascular; cytology; metabolism; Rats; Rats, Sprague-Dawley; Scavenger Receptors, Class A; metabolism
- From: Journal of Southern Medical University 2010;30(6):1250-1252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a model of smooth muscle cells differentiated from bone mesenchymal stem cells (BMSC-SMCs) in vitro and explore the relationship between scavenger receptors A (SR-A) and caveolin-1.
METHODSBMSCs were isolated from the femoral bone of SD rats by adherent culture. After treatment of the BMSC-SMCs with 80 mg/L ox-LDL for 72 h, Western blotting was performed to detect the expression of scavenger receptor SR-A, cell cholesterol transport protein ATP-binding cassette transporter Al (ABCA1) and caveolin-1.
RESULTSBMCS-SMCs became foam cells after treatment with ox-LDL. BMSC-SMC gave rise to more foam cell formation than VSMCs did. Western blotting showed that treatment with 80 mg/L ox-LDL for 72 h resulted in significantly increased expression of SR-A and significantly decreased expressions of ABCA1 and caveolin-1.
CONCLUSIONSTreatment of BMCS-SMCs with ox-LDL results in cholesterol ester accumulation in the cells to result in foam cells, the mechanism of which involves up-regulation of scavenger receptor SR-A expression and down-regulation of the reverse cholesterol transport protein ABCA1 and caveolin-1 expression.
