Short hairpin RNA targeting N-acetylglucosaminyltransferase V inhibits the proliferation of prostate cancer PC-3 cell line.
- Author:
Yong-hua YAO
1
;
Ting WEI
;
Ya-hui LI
;
Jian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Humans; Male; N-Acetylglucosaminyltransferases; genetics; Prostatic Neoplasms; genetics; pathology; RNA Interference; RNA, Small Interfering; genetics; Transfection
- From: Journal of Southern Medical University 2010;30(6):1253-1255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of short hairpin RNA (shRNA) targeting N-acetylglucosaminyltransferase V (GnT-V) on the proliferation of prostate cancer PC-3 cells.
METHODSLipofectamine 2000 was used to transfect the recombinant plasmids carrying the shRNA targeting GnT-V gene into PC-3 cells. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to measure the mRNA expression of GnT-V, and CCK-8 assay was used to measure the cell proliferation after the transfection.
RESULTSThe recombinant plasmids were successfully transfected into PC-3 cells, resulting in a reduction of GnT-V mRNA expression by 73%. The proliferation of PC-3 cells was significantly inhibited after the transfection.
CONCLUSIONThe shRNA targeting GnT-V gene can reduce the expression of GnT-V mRNA and inhibit the proliferation of PC-3 cells in vitro.
