Effect of FCGR3A Polymorphisms on Antibody-dependent Cetuximab-mediated Cytotoxicity in A549 Cells.
- Author:
Jin-yu LI
1
;
Yan-yun ZHU
1
;
Guo-qing ZHANG
1
;
Sheng-jie SUN
1
;
Shun-chang JIAO
1
Author Information
- Publication Type:Journal Article
- MeSH: Antibody-Dependent Cell Cytotoxicity; Cell Line, Tumor; Cetuximab; Genotype; Humans; Polymorphism, Genetic; Receptors, IgG
- From: Acta Academiae Medicinae Sinicae 2015;37(6):645-649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of FCGR3A polymorphisms on the antibody-dependent cell-mediated cytotoxicity (ADCC) activity induced by cetuximab against A549 cells.
METHODSA549 cell line was used as target cells and NKTm cells as effector cells. FCGR3A polymorphisms were detected by direct sequencing. The ADCC activity mediated by cetuximab was assessed by CCK-8 assay.
RESULTSThree genotypes of FCGR3A were detected:V/V,V/F,and F/F. The ADCC activity of NKTm cells with these three different genotypes mediated by cetuximab were significantly different (P=0.0015). NKTm cells with FCGR3A-158V/V genotypes had significantly higher ADCC activity than FCGR3A-V/F or F/F genotypes (P<0.01),whereas the ADCC activity between V/F and F/F genotype showed no statistical significance(P>0.05).
CONCLUSIONFCGR3A polymorphisms have an impact on ADCC activity mediated by cetuximab in NKTm cells.
