Effect of High MiR-146a Expression on the Inflammatory Reaction in BV2 Cells.
- Author:
Na ZHAO
1
;
Le SHEN
1
;
Hao-wu JIANG
2
;
Chao MA
2
;
Yu-guang HUANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line; Enzyme-Linked Immunosorbent Assay; Humans; Inflammation; Interleukin-1 Receptor-Associated Kinases; Interleukin-6; Lipopolysaccharides; MicroRNAs; RNA, Messenger; Real-Time Polymerase Chain Reaction; Signal Transduction; TNF Receptor-Associated Factor 6; Transfection; Tumor Necrosis Factor-alpha
- From: Acta Academiae Medicinae Sinicae 2016;38(1):27-32
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the effect of MiR-146a regulator function on the inflammatory response in neuroglia cell (microglia).
METHODSBV2 cells were transfected by MiR-146a mimics,and then stimulated by lipopolysaccharide (LPS). MiR-146a expression was measured by real-time polymerase chain reaction (real-time PCR). Interleukin (IL)-6 and tumor necrosis factor α (TNFα) were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, IL-1 receptor-associated kinase 1 (IRAK1) and TNF receptor-associated factor 6 (TRAF6) were detected by PCR and Western blotting.
RESULTSCompared to the normal control group, MiR-146a expression was significantly elevated by transfection with MiR-146a mimics (t=5.846, P=0.0021). The expression levels of IRAK1, TRAF6, TNFα, and IL-6 significantly increased in the LPS-stimulated BV2 cells compared to the non-stimulated BV2. The enhancement of MiR-146a resulted in significantly decreased IL-6 (t=5.200, P=0.0003) and TNFα (t=9.812, P<0.0001) secretion. The mRNA (t=5.353, P=0.0007) and protein (t=6.980, P=0.0009) levels of TRAF6, but not IRAK1, also significantly decreased.
CONCLUSIONMiR-146a may negatively suppress the inflammatory response of BV2 cells by regulating the expression of IRAF6 molecules in the TLR4 signaling pathway.
