Protection of Nerve Injury with Exosome Extracted from Mesenchymal Stem Cell.

Jun-ji WEI; Yun-fei CHEN; Chun-ling XUE; Bai-tao MA; Ya-mei SHEN; Jian GUAN; Xin-jie BAO; Hao WU; Qin HAN; Ren-zhi WANG; Chun-hua ZHAO

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Protection of Nerve Injury with Exosome Extracted from Mesenchymal Stem Cell.

Author: Jun-ji WEI ¹ ; Yun-fei CHEN ² ; Chun-ling XUE ² ; Bai-tao MA ¹ ; Ya-mei SHEN ² ; Jian GUAN ¹ ; Xin-jie BAO ¹ ; Hao WU ¹ ; Qin HAN ² ; Ren-zhi WANG ¹ ; Chun-hua ZHAO ²
Author Information

1. Department of Neurosurgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
2. Center of Excellence in Tissue Engineering,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
Publication Type:Journal Article
MeSH: Animals; Central Nervous System; injuries; Exosomes; Glutamic Acid; Humans; Mesenchymal Stromal Cells; PC12 Cells; Rats; Vascular Endothelial Growth Factor A
From: Acta Academiae Medicinae Sinicae 2016;38(1):33-36
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the protective effect of Exosomes from human adipose-derived mesenchymal stem cells (hAMSCs) in neural injury induced by glutamate and its possible mechanism.
METHODSCharacteristics of Exosomes from hAMSCs were identified by electron microscopy and Western blot analysis. Cytokines that might play a major role in the protective effect were tested by enzyme-linked immunosorbent assay (ELISA). The protective action of Exosome and its possible signaling pathway were researched by the in vitro neural injury induced by glutamate, including control group (without Glu), Glu group (dealing with Glu), Glu+Exo group (dealing with Glu +100 ng/ml Exo), Glu+Exo+Akt group (dealing with Glu+100 ng/ml Exo+10 μmol/L Akt), Glu+Exo+Erk group (dealing with 100 ng/ml Glu+100 ng/ml Exo+10 μmol/L Erk), and Glu+Exo+TrkB group (dealing with Glu+100 ng/ml Exo +10 μmol/L TrkB).
RESULTSExosomes from hAMSCs had similar sizes to those isolated from other kinds of cells, and expressed the characteristic proteins such as CD63, CD81, HSP70, and HSP90. Cytokines that had neurotrophic effects on Exosomes were mainly insulin-like growth factor and hepatocyte growth factor, with the concentration being 9336.49±258.63 and 58,645.50±16,014.62, respectively; brain derived neurotrophic factor, nerve growth factor,and vascular endothelial growth factor had lower levels, with the concentration being 1928.25±385.47, 1136.94±5.99, and 33.34±9.43, respectively. MTS assay showed that the PC12 cell survival rates were 0.842±0.047, 0.306±0.024, 0.566±0.026, 0.461±0.016, 0.497±0.003, and 0.515±0.034 in the control group, Glu group, Glu+Exo group, Glu+Exo+Akt group, Glu+Exo+Erk group, and Glu+Exo+TrkB group; obviously, it was significantly lower in Glu group than in control group (P=0.02), significantly higher in Glu+Exo group than in Glu group (P=0.01), and significantly lower in Glu+Exo+Akt group than in Glu+Exo group (P=0.01).
CONCLUSIONExosomes secreted from hAMSCs have protective effect against neuron damage induced by glutamate, which may be mediated through activating the PI3/K-Akt signalling pathway.