Human Umbilical Cord-derived Mesenchymal Stem Cells Secrete Interleukin-6 to Influence Differentiation of Leukemic Cells.
- Author:
Fang CHEN
1
;
Feng-xia MA
1
;
Yang LI
1
;
Fang-yun XU
1
;
Ying CHI
1
;
Shi-hong LU
1
;
Zhong-chao HAN
1
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cell Line, Tumor; Humans; Interleukin-6; metabolism; Leukemia, Promyelocytic, Acute; pathology; Mesenchymal Stromal Cells; metabolism; Tretinoin; pharmacology; Umbilical Cord; cytology
- From: Acta Academiae Medicinae Sinicae 2016;38(2):164-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of human umbilical cord-derived mesenchymal stem cells (UC-MSC) on the differentiation of leukemic cells.
METHODSThe co-culture system of UC-MSC with acute promyelocytic leukemic cell line NB4 cells was constructed in vitro,and the differentiation status of the leukemic cells was assessed by cell morphology,nitroblue tetrazolium reduction test,and cell surface differentiation marker CD11b.
RESULTSUC-MSC induced the granulocytic differentiation of NB4 cells. When UC-MSC and a small dose of all-trans retinoic acid were applied together,the differentiation-inducing effect was enhanced in an additive manner. Interleukin (IL)-6Ra neutralization attenuated differentiation and exogenous IL-6-induced differentiation of leukemic cells.
CONCLUSIONUC-MSC can promotd granulocytic differentiation of acute promyelocytic leukemia cells by way of IL-6 and presented additive effect when combined with a small dose of all-trans retinoic acid.
