Effect of smoking on the microRNAs expression in pneumoconiosis patients.
- Author:
Ming ZHANG
1
;
Yanrang WANG
2
;
Deyi YANG
;
Yitao LIU
;
Xin WANG
;
Jundi XIA
;
Louxin ZHANG
;
Lianhong XIE
Author Information
- Publication Type:Journal Article
- MeSH: Humans; MicroRNAs; biosynthesis; Pneumoconiosis; metabolism; physiopathology; Polymerase Chain Reaction; Smoking; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(9):686-688
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of smoking on the microRNAs (miRNAs) expression in pneumoconiosis patients.
METHODSReal-time qPCR was used to measure the expression levels of miR-21, miR-200c, miR-16, miR-204, miR-206, miR-155, let-7g, miR-30b, and miR-192 in 36 non-smoking patients with pneumoconiosis and 38 smoking patients with pneumoconiosis, and the differences in expression levels between the two groups were evaluated by two-independent samples t-test.
RESULTSThe expression of miR-192 in serum showed a significant difference between non-smoking and smoking pneumoconiosis patients (P < 0.05), and it decreased gradually in smoking patients with stage I and II pneumoconiosis. In the serum of all pneumoconiosis patients, the expression level of miR-16 was the highest, while the expression level of miR-204 was the lowest.
CONCLUSIONPneumoconiosis patients have differential expression of miRNAs in serum, and smoking has an effect on the miRNAs expression in pneumoconiosis patients.
