Inhibition effect of N-acetyl-seryl-aspartyl-lysyl-proline on myofibroblast differentiation of MRC-5 human fetal lung fibroblasts inuced by Ang II.
- Author:
Shifeng LI
1
;
Shipu DU
;
Xinxin XUE
;
Dingjie XU
;
Hong XU
;
Yue SUN
;
Haijing DENG
;
Yi YANG
;
Zhongqiu WEI
;
Jingrui TIAN
;
Fang YANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Actins; Angiotensin II; Cell Differentiation; drug effects; Collagen; Collagen Type I; Cyclic AMP; analogs & derivatives; Fetus; cytology; Fibroblasts; cytology; Guanine Nucleotide Exchange Factors; Humans; Lung; cytology; Myofibroblasts; drug effects; Oligopeptides; pharmacology; Serum Response Factor; Trans-Activators
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(11):801-805
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the inhibition effect of N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) on myofibroblast differentiation of MRC-5 human fetal lung fibroblasts induced by angiotensin (Ang) II.
METHODSThe study was divided into 2 step: (1) MRC-5 human fetal lung fibroblasts was induced for 48 h at different dose of Ang II and at different time point by 100 nmol/L Ang II. Then the expression of collagen type I and α-smooth muscle actin (α-SMA) were mesaured by western blot. (2) MRC-5 human fetal lung fibroblasts were divided into 4 group: (1) control, (2) Ang II, (3) Ang II+Ac-SDKP, (4) Ang II+8-Me-cAMP (a specific activator of Epac). The α-SMA expression was observed by immnocytochemical stain. The protein expression of collagen type I, α-SMA, serum response factor (SRF), myocardin-related transcription factor (MRTF)-A, exchange protein directly activated by cAMP (Epac) 1, 2 were measured by Westen blot.
RESULTSMyofibroblast differentiation could be induced by Ang II from MRC-5 cells with a dose- and time-dependent manner. The up-regulation of SRF and MRTF-A were observed in MRC-5 cells induced by Ang II and accompanied with collagen I and α-SMA increased. Pre-treatment with 8-Me-cAMP or Ac-SDKP could attenuated all this changes induced by Ang II, and promoted the expression of Epac1.
CONCLUSIONAc-SDKP can inhibit the myofibroblast differentiation of MRC-5 cells induced by Ang II via Epac1 activating.
