Typing and identification of non-polio enterovirus from acute flaccid paralysis cases in Ningxia,1997-2011
10.3760/cma.j.issn.0254-6450.2012.11.015
- VernacularTitle:宁夏地区1997-2011年急性弛缓性麻痹病例非脊髓灰质炎肠道病毒分型鉴定分析
- Author:
Jiang-Tao MA
1
;
Hui CHEN
;
Fang YUAN
;
Xue-Min MA
;
Guang-Yu GUAN
;
Jun ZHAN
Author Information
1. 宁夏回族自治区疾病预防控制中心
- Keywords:
Non-polio enterovirus;
Molecular identification;
Epidemiology
- From:
Chinese Journal of Epidemiology
2012;33(11):1163-1166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the serotype of 73 non-polio enterovirus (NPEV) strains from acute flaccid paralysis (AFP) cases in Ningxia province,during 1997-2011.Methods Partial sequencing of the VP1 region was amplified by RT-PCR with degenerate primers and sequenced while sequences were compared with the database of GenBank by the BLAST algorithm.Evolution was analyzed by constructing phylogenetic tree using Mega 5.1.Results In this study,a total of 73 NPEVs were analyzed,including 4 strains un-typed,69 strains typed by RT-PCR.A total of 27 serotypes were identified,including 8 serotypes of human enterovirus (HEV)-A,19 serotypes of HEV-B.The HEV-B group (46/69,66.7％) constituted the largest proportion of isolates,followed by HEV-A (23/69,33.3％),but no strains were found that belonged to HEV-C or HEV-D group.In the 69 strains,enterovirus 71 was the most frequently seen isolates,followed by coxsackie-virus A4,16,9 and echovirus 24,6.Conclusion HEV-B was the most predominant (46/69,66.7％) serotype of NPEV in Ningxia during the AFP surveillance,in 1997-2011.
