Emodin enhances antitumor effect of gemcitabine in model of SW1990 cell xenograft on athymic mouse.
- Author:
Weitian WEI
1
;
Yafei GUO
;
Hui CHEN
;
Dianlei LIU
;
Hongchun GUO
;
Shengzhang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Cell Transformation, Neoplastic; Cytochromes c; metabolism; Deoxycytidine; analogs & derivatives; pharmacology; Drug Synergism; Emodin; pharmacology; Female; Gene Expression Regulation, Neoplastic; drug effects; Humans; Mice; Mice, Nude; Proto-Oncogene Proteins c-bcl-2; metabolism; Tumor Burden; drug effects; Xenograft Model Antitumor Assays; bcl-2-Associated X Protein; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(24):3348-3353
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the enhanced effect of gemcitabine by emodin and the possible mechanisms of the enhancement.
METHODBased on the model of SW1990 cell xenograft on athymic mouse, the mice were randomized to four groups with intraperitoneal (IP) injections of different drugs: group N (injecting 0.9% sodium chloride), group E (emodin, 40 mg x kg(-1)), group G (gemcitabine, 125 mg x kg(-1)), and group E + G (emodin 40 mg x kg(-1) and gemcitabine 80 mg x kg(-1) in combination). The tumor volume, tumor weight and body weight of mice were measured during the drug therapy. The mice were sacrificed one week after last injection of drug. Tunel assay were used used to detect the apoptosis of tumor cells. And immunohistochemistry (IHC) and Western blot (WB) were used to detect the variance of the apoptosis relative protein expression of Bax, Bcl-2, and Cytochrome C .
RESULTOne week after the last administration, the mean tumor volume and tumor weight in group E + G were significantly decreased compared to the other groups. Tunel assay showed group E + G presented apparently more apoptosis than the other groups. Immunohistochemistry (IHC) and Western blot (WB) analysis showed the expression of Cytochrome C in cytoplasmin and Bax in group E + G was apparently upregulated while the expression of Bcl-2 was apparently downregulated compared to the other groups. As a result, Bcl-2/Bax ratio was significantly decreased in group E + G.
CONCLUSIONEmodin can significantly improve the antitumor effect of gemcitabine on transplanted tumor of SW1990 cell line through apparently enhancing the tumor cell apoptosis by gemcitabine. Downregulation of Bcl-2/Bax ratio and promoting release of Cytochrome C from mitochondria is possibly one of the mechanisms of the augmented apoptosis.
