Study total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes.
- Author:
Huiyu XU
1
;
Zhiwei CHEN
;
Li ZHOU
;
Jianzhao NIU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; genetics; Caspases; genetics; Dose-Response Relationship, Drug; Gene Expression Regulation, Neoplastic; drug effects; Glucosides; pharmacology; Humans; K562 Cells; Paeonia; chemistry; Signal Transduction; drug effects; Time Factors
- From: China Journal of Chinese Materia Medica 2010;35(24):3377-3381
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes.
METHODBy MTT and flow cytometric, real-time quantitatie polymerase chain reaction (PCR) and Western blot methods researched the level of genes and proteins.
RESULTThe total glucosides of Radix Paeoniae Rubra could inhibit K562 cell growth by MTT method, there was the relationship of concentration-time between them, TGC prompted K562 cell translocation of phosphatidylserine, may be apoptosis through non-receptor-dependent pathways because caspase-3 mRNA, caspase-9 mRNA and cytochrome C increased, caspase-8 mRNA had no significant change. The expression of Bcl-2 protein and Bcl-X1 protein could decreased, the expression of Bax protein could increased by regulating gene expression.
CONCLUSIONTGC induced K562 cell apoptosis that might be through the mitochondria pathway, when cell apoptosis occured, Bcl-2 or Bcl-XI proteins combination of Bax protein would be displacement, then the mitochondrial membrane became permeable to release a series of material, then lead to cell death.
