Study on absorption ingredients of Plantaginis semen by in vitro everted intestinal sac method.
- Author:
Qin TAN
1
;
Jingjing ZHU
;
Weihao WANG
;
Zhimin WANG
;
Jiucheng CUI
;
Jingjing KONG
;
Meng QI
;
Li YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Body Fluids; chemistry; metabolism; Drugs, Chinese Herbal; pharmacokinetics; Ileum; chemistry; drug effects; metabolism; Intestinal Absorption; Jejunum; chemistry; drug effects; metabolism; Male; Models, Biological; Plantago; chemistry; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2011;36(2):150-153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for quick investigating the absorption ingredients of Plantaginis semen and guiding the index selection for its quality control.
METHODThe absorption of three concentrations of Plantaginis semem was investigated with the in vitro everted intestinal sac (VEIS) model The intestinal sac contents of jejunum and ileum were collected at different time and geniposidic acid was detected by HPLC and LC-MS(n) as the representative marker.
RESULTSix ingredients could be detected. At different concentrations of Plantaginis semen, geniposidic acid tested by VEIS showed that there was a good linear correlation between the drug absorption from the medium across the intestinal epithelium into the sac contents in various intestines section. The absorption of the gut sacs from 0 to 90 min manifested a significant time-dependent manner. The Ka of geniposidic acid in the jejunum and ileum increased along with the raised dosage of the Plantaginis Semen (P < 0.05), which indicated a passive absorption manner.
CONCLUSIONThis method can be used as a tool to investigate the absorption ingredients of Plantaginis Semen. Comparing with the jejunum, the ileum can provide more absorption information faster. The optimal incubation time in intestinal sac was 90 min.