Optimization of extraction procedure of tongmai granules by orthogonal design with pharmacodynamic index.
- Author:
Xiaobin JIA
1
;
Yang HUANG
;
Bin CHEN
;
Qi LIU
;
Zhongwen SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; isolation & purification; pharmacokinetics; Male; Rats; Rats, Sprague-Dawley; Technology, Pharmaceutical; instrumentation; methods
- From: China Journal of Chinese Materia Medica 2011;36(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the extraction technology for anti-myocardial ischemia active component group in tongmai granules.
METHODThe extracting conditions were optimized by using orthogonal experiment and pharmacodynamic index. Employing puerarin, ferulic acid, tanshinone IIA, and salvianolic acid B as indexes, the effects of ethanol concentration, solvent volume, extraction times and extraction time on the extracting process were investigated.
RESULTThe optimum extraction process was adding 8 times amount of 70% alcohol, refluxing and extracting for 3 times, 1.5 h each time.
CONCLUSIONThis extraction process shows good stability and is available for extracting anti-myocardial ischemia component group in tongmnai granules.
