Study on fragmentation of vitexin and isorhamnetin-3-O-beta-D-rutinoside using electrospray quadrupole time of flight mass spectrometry.
- Author:
Kunping LI
1
;
Chongkai GAO
;
Weiming LI
Author Information
- Publication Type:Journal Article
- MeSH: Apigenin; chemistry; Disaccharides; chemistry; Drugs, Chinese Herbal; chemistry; Flavonoids; chemistry; Molecular Structure; Spectrometry, Mass, Electrospray Ionization; instrumentation; methods
- From: China Journal of Chinese Materia Medica 2011;36(2):180-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the fragmentation pathway of vitexin and isorhamnetin-3-O-beta-D-rutinoside with CID-TOF-MS.
METHODEquipped with an LC-MS was carried out using an ultra-performance liquid chromatography, electrospray ionization quadrupole collision-induced dissociation-TOF-MS.
RESULTESI-MS spectrum showed [M-H]- base peak of m/z 431. 0958 and m/z 623.1566. The CID-MS of vitexin showed five basic fragment ions, three of which corresponded to the glucosyl ring fracture: m/z 353, 341 and 311; other two were benzyl ion m/z 283, aglycone ion m/z 269. In addition, two low abundance ions, namely, m/z 161 and m/z 117, generated by RDA cracking ions, were also characteristic ions. The CID-MS of isorhamnetin-3-O-beta-D-rutinoside showed six main characteristic fragments ions corresponding to the loss of rhamnosyl m/z 477 and the glycosyl ring fracture: m/z 387, 357 and 311, and aglycone ion m/z 315. In addition, B ring generated m/z 300 and m/z 271 and C ring generated m/z 243 and the RDA cleavage generated m/z 151 and m/z 125.
CONCLUSIONThose fragment ions can be used to quickly identify vitexin and isorhamnetin-3-O-beta-D-rutinoside.
