Effect of jinqiaomai on expression of TLR2/4, MyD88 mRNA and IkappaB-alpha in lung tissue of rats with Klebsiella pneumonia.
- Author:
Liuyi DONG
1
;
Chunyan WANG
;
Changqing WU
;
Qin JIANG
;
Zhifen ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Gene Expression; drug effects; Humans; I-kappa B Proteins; genetics; metabolism; Klebsiella Infections; drug therapy; genetics; metabolism; microbiology; Klebsiella pneumoniae; drug effects; physiology; Lung; drug effects; metabolism; microbiology; Male; Myeloid Differentiation Factor 88; genetics; metabolism; NF-KappaB Inhibitor alpha; Random Allocation; Rats; Rats, Sprague-Dawley; Toll-Like Receptor 2; genetics; metabolism; Toll-Like Receptor 4; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2011;36(2):200-204
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the mechanism of protective effect of Jinqiaomai (JQM) on lung injury induced by Klebsiella pneumonia in rats.
METHODThe model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, JQM (6, 3, 1.5 g x kg(-1)) three groups, levofloxacin (25 mg x kg(-1)) group, JQM (3 g x kg(-1)) + levofloxacin (25 mg x kg(-1)) group. The contents of IL-1beta, ICAM-1 and INF-gamma in the lung tissue homogenate were measured by radio-immunoassay and Elisa. TLR2/4 mRNA and MyD88 mRNA expression were detected by RT-PCR. IkappaB-alpha expression was detected by Western Blot.
RESULTThe rats of model group had obvious lung injury, but those of JQM, JQM + levofloxacin and levofloxacin groups had less injury. The contents of IL-1beta, ICAM-1 and INF-gamma in lung tissue homogenate and the expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha in lung of model group were significantly higher than those in the control group (P < 0.05 or P < 0.01), while IL-1beta, ICAM-1 and INF-gamma of JQM groups were significantly lower than those of model group (P < 0.05 or P < 0.01). The expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha of JQM (6.3 g x kg(-1)) groups were significantly lower than those of model group(P <0. 05 or P <0. 01).
CONCLUSIONThe lung injury induced by Klebsiella pneumonia is related to TLR2/4, MyD88 mRNA and IkappaB-alpha. To decrease the excessive expression of TLR2/4, MyD88 mRNA and IkappaB-alpha might be the main mechanism of protective effect of Jinqiaomai on lung injury.
