Classification of Pueraria lobata in different geographical regions.
- Author:
Dongji LIU
1
;
Zhikui YU
;
Chunsheng LIU
;
Xueyong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA, Ribosomal Spacer; Genes, Mitochondrial; Genotype; Molecular Sequence Data; Mutation; Pueraria; classification; genetics
- From: China Journal of Chinese Materia Medica 2011;36(3):299-301
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo classify Pueraria lobata originated from different geographical regions based on ITS,psbK-psbI and trnH-psbA information.
METHODTwenty-four samples of P. lobata were collected from northeast China, north China, central China and northwest China. DNA extraction, PCR, sequence and genotypes/haplotypes analysis were performed .
RESULTITS1, 5.8S and ITS2 varied only 1 bp respectively, psbK-psbI 2 bps; trnH-psbA varied 1 bp and 10 bp deletion.
CONCLUSIONBased on the variation of ITS,psbK-psbI and trnH-psbA, 4 genotypes and 2 haplotypes were identified, respectively.
