AFLP analysis of genetic diversity of Alpinia officinarum.
- Author:
Quan YANG
1
;
Chunrong ZHANG
;
Hubiao CHEN
;
Xifeng TENG
;
Xiaomin TANG
Author Information
- Publication Type:Journal Article
- MeSH: Alpinia; classification; genetics; Amplified Fragment Length Polymorphism Analysis; Genetic Markers; Genetic Variation; Genotype; Phylogeny
- From: China Journal of Chinese Materia Medica 2011;36(3):330-333
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the genetic diversity and relationship of different Alpinia officinarum germplasm.
METHODAmplified fragment length polymorphism (AFLP) markers were developed to analyze genetic polymorphism in A. officinarun from eight resources. The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A. officinarum germplasm and to construct the genetic phylogenetic tree.
RESULTA total of 1,120 fragments were genotyped using AFLP with eight prime combinations. Analysis identified 1,044 polymorphic fragments, accounting for 92.57% of the total detected variation. Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A. officinanrum.
CONCLUSIONSignificant polymorphism and genetic diversity can be observed among A. officinarum germplasm resources.
