Analysis of genetic diversity of gemplasm of Pinellia ternata based on SRAP.
- Author:
Min YANG
1
;
Keli CHEN
Author Information
- Publication Type:Journal Article
- MeSH: DNA Fingerprinting; Ecosystem; Genetic Variation; Geography; Phylogeny; Pinellia; classification; genetics; Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2011;36(3):334-337
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess population genetic diversity of Pinellia ternata with different phenotype and from different habitat by sequence-related amplified polymorphism (SRAP) technique.
METHODFourteen appropriate primer pairs were selected out from a total of 80 pairs for SRAP PCR amplification. A Jaccard's genetic similarity matrix and a dendrogram were established using SPSS 16.0 software.
RESULTThe 14 primer pairs could amplify 171 bands, and the percentage of polymorphic bands was 15.8%. Based on the dendrogram, P. ternata in the same habitat clustered in a clade.
CONCLUSIONThese results suggested that SRAP markers could be used as an effective molecular technique for the diversity study of P. ternata and the habitat was more important than the phenotype in identification of P. ternata germplasm resource.
