Analysis on chloroplast DNA sequences of Polygonum capitatum of different geographical population.
- Author:
Yanlei JIN
1
;
Tao ZHOU
;
Liyan ZHANG
;
Weike JIANG
;
Shenghua WEI
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Base Sequence; DNA, Chloroplast; genetics; Molecular Sequence Data; Mutation; Phylogeny; Polygonum; classification; genetics; Sequence Alignment; Sequence Analysis, DNA
- From: China Journal of Chinese Materia Medica 2011;36(3):342-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the variation of chloroplast DNA gene sequences and the geographical origins of Polygonum capitatum in order to provide the molecular evidence for its excellent germplasm resources.
METHODPCR direct sequencing was applied to detect the chloroplast psbA-trnH, trnL-trnF gene sequence of 11 samples collected from 11 populations of P. capitatum.
RESULTThe psbA-trnH gene sequence of P. capitatum from different populations was 402 bp in length, there were 6 variable sites. TrnL-F gene sequence was 875 bp, there were 5 variable sites. The clusters diagram by UPGMA method showed that P. capitatum groups in Yunnan and Guizhou existed a considerable variation.
CONCLUSIONP. capitaturni which is located in the east of Yunnan and the west of Guizhou is helpful of screening the germplasm resources.
