Preliminary functional analysis of intron in chalcone synthase gene from Scutellaria baicalensis.
- Author:
Chong WU
1
;
Luqi HUANG
;
Yuan YUAN
;
Nan ZHANG
;
Shufang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Acyltransferases; genetics; Base Sequence; Gene Expression Regulation, Plant; drug effects; Gene Order; Genetic Vectors; Introns; Molecular Sequence Data; Plants, Genetically Modified; genetics; metabolism; Polyethylene Glycols; pharmacology; Regulatory Sequences, Nucleic Acid; genetics; Scutellaria baicalensis; enzymology; genetics; Sequence Alignment; Tobacco; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2011;36(3):361-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the function of the chalcone synthese gene introns in Scutellaria baicalensis, and clarify preliminarily their role in abiotic stress.
METHODThe CHS introns with specific primers were cloned and bioinformatic method was applied to predict the cis-elements in the intron of CHS. The introns were subcloned into binary vector, pCAMBIA-1301 before being transferred to tobacco. Then the activity of GUS of the transgenic tobacco seeds was analyzed.
RESULTSeven cis-elements were found in the introns. Under the dark and high temperature GUS expression rose at the first (3 h), but then declined (9 h). ABA and MeJA regulated insignificantly the GUS activity in normal temperature; treatment of 10% PEG induced GUS expression.
CONCLUSIONCHS introns could be play a role in the regulation of S. baicalensis phenylpropanoid biosynthetic pathway.
