The change in IL-13 mRNA expression in rat lungs with acute pulmonary injury induced by lipopolysaccharide.

Qi LI; Guisheng Qian; Qing Zhang; Jianyue XU; Yong Long; Zhengcai Tang; Jinqian Gong

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The change in IL-13 mRNA expression in rat lungs with acute pulmonary injury induced by lipopolysaccharide.

Author: Qi LI ¹ ; Guisheng QIAN ; Qing ZHANG ; Jianyue XU ; Yong LONG ; Zhengcai TANG ; Jinqian GONG
Author Information

1. Institute of Respiratory Disease, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China.
Publication Type:Journal Article
MeSH: Acute Disease; Animals; Disease Models, Animal; Female; Interleukin-13; biosynthesis; genetics; Lipopolysaccharides; Male; RNA, Messenger; biosynthesis; Rats; Rats, Wistar; Respiratory Function Tests; Respiratory Insufficiency; chemically induced; metabolism; physiopathology
From: Chinese Journal of Burns 2002;18(3):145-148
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo observe the change in IL-13 mRNA expression in rat lungs with acute lung injury (ALI) induced by lipopolysaccharide (LPS), so as to investigate the significance of anti-inflammatory mechanism in the development of ALI.
METHODS(1) Different doses (2 approximately 8 mg/kg) of LPS were injected via the tail vein to the rats to establish ALI model. (2) The IL-13 mRNA content in pulmonary tissue was determined by RT-PCR.
RESULTS(1) Different degrees of ALI was induced in the rats by different doses of LPS. ARDS was produced in the rats by LPS of 6 mg/kg. (2) The IL-13 mRNA expression in the pulmonary tissue could be enhanced by the induction of LPS, especially when its dose was larger than 6 mg/kg.
CONCLUSION(1) Rat ALI model could be produced by LPS injection via the tail vein. (2) LPS in dose of 6 mg/kg was the threshold for the production of rat ARDS. (3) The incidence of rat ARDS might be closely related to the enhanced expression of IL-13 mRNA in rat pulmonary tissue.