Changes of the mRNA expression and the intestinal mucosal cyclooxygenase activity in scalded rats.
- Author:
Desheng MENG
1
;
Shiliang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; enzymology; genetics; pathology; Cyclooxygenase 1; Cyclooxygenase 2; Female; Gene Expression; Intestinal Mucosa; enzymology; Isoenzymes; biosynthesis; genetics; metabolism; Male; Membrane Proteins; Prostaglandin-Endoperoxide Synthases; biosynthesis; genetics; metabolism; RNA, Messenger; biosynthesis; Rats; Rats, Wistar
- From: Chinese Journal of Burns 2002;18(4):210-212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of the mRNA expression and the intestinal mucosal cyclooxygenase (COXs) activity in scalded rats.
METHODSWistar rats inflicted with 30% TBSA III degree scalding were employed as the model. The changes of COX-1, COX-2 activities were determined by substrate fluorescence analysis and the mRNA expressions of COX-1 and COX-2 by RT-PCR.
RESULTSThe mRNA expressions and the activities of COX-2 in rat intestinal mucosa increased obviously after injury. But those of COX-1 exhibited lower range of change.
CONCLUSIONThe pathological mechanism of rat intestinal mucosa injury after scalding might be closely related to the COXs participation by different styles between the two enzymes.
