The influence of lanthanum chloride on the TNFalpha expression of murine peritoneal macrophages stimulated by lipopolysaccharide.
- Author:
Yang WANG
1
;
Keng YUAN
;
Yong CAO
;
Guohui LI
;
Yucheng DAI
;
Shuilian ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Female; Gene Expression Regulation; drug effects; Lanthanum; pharmacology; Lipopolysaccharides; pharmacology; toxicity; Macrophages, Peritoneal; cytology; drug effects; metabolism; Male; Mice; Mice, Inbred BALB C; RNA, Messenger; drug effects; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; genetics; secretion
- From: Chinese Journal of Burns 2002;18(2):102-104
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the influence of lanthanum chloride on the TNFalpha expression of murine peritoneal macrophages stimulated by lipopolysaccharide (LPS).
METHODSMurine peritoneal macrophages (Mphi) were isolated, cultured and then stimulated by LPS. The influence of lanthanum chloride on the TNFalpha secretion and TNFalphamRNA expression of murine Mphi stimulated by LPS was determined by ELISA method and SYBR green fluorescence quantitative RT-PCR. Forty BALB/C mice were randomly divided into two groups and were treated by lethal dose of LPS and lanthanum chloride processed LPS, respectively. The mortality within 7 days was observed.
RESULTSThe TNFalpha secretion and TNFalphamRNA expression level of the Mphi from mice treated by lanthanum chloride processed LPS were obviously lower than those by LPS only (P < 0.01). The mortality of the mice treated by lethal dose of LPS which has been processed by lanthanum chloride was significantly lower than that by lethal dose of LPS only.
CONCLUSIONLanthanum chloride possessed the capacity of lowering down the toxicity of LPS and inhibiting the TNFalpha secretion and TNFalphamRNA expression in murine Mphi stimulated by LPS.
