An experimental study on the repair of full skin loss of rabbits with composite chitosan artificial skin.
- Author:
Yuangang LU
1
;
Jinjin WU
;
Tangyou ZHU
;
Lang HU
;
Ping HE
;
Wenwei LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; surgery; Cells, Cultured; Chitin; analogs & derivatives; metabolism; Chitosan; Dermatologic Surgical Procedures; Humans; Keratinocytes; cytology; metabolism; Male; Rabbits; Skin; injuries; Skin Transplantation; methods; Skin, Artificial; Transplantation, Heterologous; Wound Healing
- From: Chinese Journal of Burns 2002;18(1):19-22
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the practicability of repair of full skin loss of rabbits with composite chitosan artificial skin.
METHODSDermal substitute was prepared aseptically by mixing fibroblasts with composite dermal matrix gel. Keratinocytes were then seeded on the substitute and submersion - cultured thereafter for 1 week in keratinocyte culture medium. The composite was further cultured for 1 approximately 2 weeks on the surface of the culture liquid to form artificial skin. The composite chitosan artificial skin was then grafted onto the full skin loss wound of rabbits. Histological changes were undertaken periodically by tissue sampling from the grafted wound. The systemic reaction of rabbits to the artificial skin was observed.
RESULTSAll the grafted wounds healed very well without any suppuration, bleeding or infection under the grafted skin. No obvious immune rejection was seen. The artificial skin could cover the wounds for a long time with good elasticity and easy to be manipulation.
CONCLUSIONThe composite chitosan artificial skin could be an optimal biological dressing with good histocompatibility and easy to be manipulation.
