A study of the expression of hypertrophic scar related cytoskeletal genes during early postburn stage.
- Author:
Bing MA
1
;
Jun WU
;
Shaoxuan YI
;
Zhenxiang WANG
;
Weifeng HE
;
Jin ZHU
;
Gaoxing LUO
;
Xiwei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Burns; genetics; Child; Child, Preschool; Cicatrix, Hypertrophic; genetics; Cytoskeleton; genetics; Gene Expression Profiling; Humans; Male; Oligonucleotide Array Sequence Analysis; RNA, Messenger; genetics; metabolism; Tropomyosin; genetics; Up-Regulation
- From: Chinese Journal of Burns 2002;18(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the hypertrophic scar related cytoskeletal genes during early postburn stage, so as to explore their roles in postburn scar contraction.
METHODScDNA microarray chips containing 4096 human cDNAs were employed to investigate the cytoskeletal gene expression of the scar samples from human postburn hypertrophic scar. Furthermore, the expression of one of the cytoskeletal genes in hypertrophic scar tissue was studied by in situ hybridization.
RESULTSThirteen up - regulated cytoskeletal genes in 3 early postburn hypertrophic scar samples were identified. Moreover, the cells expressing human tropomyosin TM30 mRNA, one of the up - regulated cytoskeletal genes, were found increased in the early postburn hypertrophic scar samples.
CONCLUSIONIn this study up - regulated expression of many hypertrophic scar related cytoskeletal genes was found in the scar samples during early postburn stage, and they might be important factors leading to postburn hypertrophic scar formation and contraction.
