The role of C5a in adhesion properties of polymorphonuclear leukocyte to pulmonary vascular endothelial cells in burn patients with acute lung injury.
- Author:
Fenglin LU
1
;
Xihua ZHU
;
Chengxiang HU
;
Yunhui HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Antibodies, Monoclonal; pharmacology; Antigens, CD; immunology; Burns; blood; complications; Cell Adhesion; drug effects; Cells, Cultured; Complement C5a; pharmacology; Dose-Response Relationship, Drug; Endothelium, Vascular; cytology; drug effects; Fetus; Humans; Lung; Lung Diseases; complications; Neutrophils; cytology; drug effects; enzymology; Peroxidase; antagonists & inhibitors; drug effects; metabolism; Receptor, Anaphylatoxin C5a; Receptors, Complement; immunology
- From: Chinese Journal of Burns 2002;18(6):358-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the postburn adhesion properties of polymorphonuclear leukocyte (PMN) onto pulmonary vascular endothelial cells (PVEC) in burn patients with acute lung injury (ALI), so as to determine the role of C5a on PVEC-PMN adhesion.
METHODSMicrotubule sucking technique was employed to determine the PVEC-PMN adhesion. The myeloperoxidase (MPO) was also assayed to reflect the magnitude of PVEC-PMN adhesion.
RESULTSThe magnitude of PVEC-PMN adhesion increased and the adhesion force increased along with an increase in rh-C5a concentration. Simultaneously, the MPO activity was increased, which could be inhibited by anti-C5aR McAb in a concentration 1:104.
CONCLUSIONBoth C5a and C5aR participated in PVEC-PMN adhesion, which might be important in the pathogenesis of ALI.
