An experimental study on the role of indirect antigen presentation pathway on the immunogenetic of epidermal cells.
- Author:
Xi-Wei CHEN
1
;
Jun WU
;
Qi-Zhi LUO
;
Gao-Xing LUO
;
Xi-Hua WANG
;
Shao-Xuan YI
;
Wei-Feng HE
;
Bing MA
;
Ru-Peng WANG
;
Jin TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Antigen Presentation; immunology; physiology; Antigens, CD; Antigens, Differentiation; genetics; immunology; CTLA-4 Antigen; Cell Division; immunology; Cells, Cultured; Coculture Techniques; Epidermis; cytology; immunology; metabolism; Genetic Vectors; genetics; Humans; Leukocytes, Mononuclear; cytology; immunology; Lymphocytes; cytology; immunology; Signal Transduction; Transfection
- From: Chinese Journal of Burns 2003;19(1):15-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of indirect antigen presentation pathway on the immunogenecity of epidermal cells.
METHODSHuman epidermal cells (HEC), allogeneic human peripheral blood lymphocytes (PBL) and mononuclear cells (PBM, including monocytes) were isolated and cultured in vitro. HECs were transfected by human-originated CTLA4Ig-adenovirus vector. The CTLA4Ig expression was observed. Allogeneic PBLs or PBMs were added to the transfected and non-transfected HECs with simple cultured PBLs and PBMs as the control. The proliferation of PBL and PBM was determined by (3)H-TdR incooperation.
RESULTSHECs could be successfully transfected by CTLA4Ig-adenovirus vector and expressed corresponding proteins. The non-transfected HECs could stimulate slight proliferation of allogeneic PBLs (P < 0.05) and stimulate remarkable proliferation of PBMs (including monocytes) (P < 0.05). The proliferation reaction of PBLs and PBMs decreased significantly (P < 0.05) after being stimulated by HEC which was modulated by CTLA4Ig genes.
CONCLUSIONIndirect antigen presentation pathway might play important roles in the HEC immunogenicity which could be evidently inhibited by CTLA4Ig.
