Effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells.
- Author:
Jun CHEN
1
;
Ao TANG
;
Ze-Zhi WU
;
Jian QIN
;
Guang-Xia XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Actins; analysis; Animals; Burns; blood; Cell Line; Culture Media; pharmacology; Cytoskeletal Proteins; drug effects; metabolism; Elasticity; drug effects; Epithelial Cells; cytology; drug effects; metabolism; Immunohistochemistry; Intestinal Mucosa; cytology; drug effects; metabolism; Male; Rats; Rats, Wistar; Tubulin; analysis; Viscosity; drug effects
- From: Chinese Journal of Burns 2003;19(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells.
METHODSThe rat intestinal epithelial cell strain (IEC-6) was cultured and stimulated by burn serum. The changes of IECs before and after the stimulation were dynamically observed by cytoskeleton immunohistochemistry, ELISA and the measurement of cytomembranous viscoelasticity.
RESULTSDuring the early stage of burn serum stimulation, the skeleton protein expression in IEC decreased obviously with weakened positive signals of microfilaments and microtubules and with decreased cellular elasticity.
CONCLUSIONThe cytoskeleton injury could cause the increase of cellular fragility and the decrease of the viscoelasticity, which ultimately lead to the change of cellular biodynamics. These changes might directly participate the development of postburn intestinal epithelial injury.
