Characterization of the mRNA profile in ejaculated spermatozoa from healthy fertile men.
- Author:
Yang-Xing ZHAO
1
;
Qiao-Li LI
;
Zhao-Xia WANG
;
Yi-Fei WANG
;
Lian-Yun WANG
;
Zhong-Dong QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Ejaculation; Expressed Sequence Tags; Gene Expression Profiling; Humans; Male; RNA, Messenger; genetics; Spermatozoa; physiology
- From: National Journal of Andrology 2006;12(10):900-903
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the complexity of mRNA in the ejaculated sperm from healthy fertile men.
METHODSSemen samples were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis.
RESULTSA totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively.
CONCLUSIONThere exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.
