Molecular identification of Ca2+ channels in human ejaculated sperm.
- Author:
Lu LI
1
;
Ji-Hong LIU
;
Jia-Gui LI
;
Zhang-Qun YE
;
Xiu-Lai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Calcium; metabolism; Calcium Channels; biosynthesis; classification; genetics; Humans; Image Processing, Computer-Assisted; Male; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Spermatozoa; metabolism
- From: National Journal of Andrology 2006;12(10):910-912
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the types of voltage-dependent Ca2+ channels (VDCCs) in human ejaculated sperm using molecular technology.
METHODSBased on the WHO criteria, the donated semen was screened by computer-aided sperm analysis (CASA), the sperm optimized by swim-up method, and the VDCCs' alpha1 subunits examined by reverse transcription polymerase chain reaction (RT-PCR).
RESULTSSuch mRNA messages as alpha1H, alpha1G, alpha1E, alpha1B and alpha1C were detected, but alpha1A and alpha1D were not.
CONCLUSIONHuman motile sperms express multiple VDCC RNAs, among which T-type and non-L-type channel messages are likely to be predominantly expressed. Not only T-type but also non-L-type calcium channels may be major gates for the external calcium influx, which helps regulate sperm motility and acrosome reaction.
