Type IV collagenase expression in human prostate carcinoma cell lines with different metastasis potentials.
- Author:
Lin-Lin ZHANG
1
;
Da-Lin HE
;
Xiang LI
;
Lei LI
;
Guo-Dong ZHU
;
Xin-Yang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Male; Matrix Metalloproteinase 2; biosynthesis; metabolism; Matrix Metalloproteinase 9; biosynthesis; metabolism; Neoplasm Invasiveness; Neoplasm Metastasis; Prostatic Neoplasms; enzymology; pathology
- From: National Journal of Andrology 2006;12(10):913-916
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation between type IV collagenase expression and metastatic potential of human prostate carcinoma cells.
METHODSHuman prostate carcinoma cell lines (LNCaP and C4-2B) with different metastatic potentials were selected. Using SABC immunocytochemical staining and zymographic analysis, we detected the difference of type IV collagenase expression and activity between the two cell lines.
RESULTSThe expression of type IV collagenase (MMP-2, MMP-9) could be detected in both LNCaP and C4-2B, mainly in cytoplasm, much higher in C4-2B than in LNCaP (P < 0.01). The activities of type IV collagenase in the conditioned medium of prostatic carcinoma cell LNCaP was the lowest, and the quantitative estimation values of MMP-2 and MMP-9 were 0.89 +/- 0.02 and 0.86 +/- 0.01, respectively. However, the quantitative estimation values of MMP-2 and MMP-9 in the conditioned medium of C4-2B were 96.32 +/- 4.36 and 33.89 +/- 1.84, respectively. Compared with LNCaP, the activity of type IV collagenase in C4-2B was much higher (P < 0.01).
CONCLUSIONCompared with LNCaP, the higher production capability of type IV collagenase in C4-2B may possibly contribute to its invasive and metastatic potentials. The expression of type IV collagenase of human prostate carcinoma cells is closely correlated to the metastatic and invasive potential.
