Phosphodiesterase type 5 siRNA increases cGMP in the smooth muscle cells of human corpus cavernosum.
- Author:
Guo-qiang CHEN
1
;
Wen-jun BAI
;
Xiao-feng WANG
;
Shi-jun LIU
;
Pei-ying HE
;
Shu-kun HOU
Author Information
- Publication Type:Journal Article
- MeSH: 3',5'-Cyclic-GMP Phosphodiesterases; genetics; Cells, Cultured; Cyclic GMP; metabolism; Cyclic Nucleotide Phosphodiesterases, Type 5; Gene Silencing; Humans; Male; Myocytes, Smooth Muscle; drug effects; metabolism; Penis; metabolism; RNA, Small Interfering; pharmacology; Transfection
- From: National Journal of Andrology 2006;12(11):979-984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of phosphodiesterase type 5 (PDE5) small interfering RNA (siRNA) on the cGMP in the smooth muscle cells of human corpus cavernosum, and to provide an experimental groundwork for the gene therapy of erectile dysfunction (ED).
METHODSSmall interfering RNAs targeting PDE5 gene were synthesized by using web design software provided by Ambion, three siRNAs and a control siRNA were synthesized by Ambion. siRNAs were transfected into the smooth muscle cells of human corpus cavernosum by using siPORT Lipid reagent. cGMP was detected by ELISA at different times (24, 48, 72 and 96 h) after transfection.
RESULTSThe cGMP levels of the siRNA1, siRNA2 and siRNA3 groups were significantly higher than those of the siRNA control and blank control groups (P < 0.05), and so was it in the siRNA1 group than the siRNA2 and siRNA3 groups (P < 0.05), with significant difference between the siRNA control and the blank control group (P > 0.05).
CONCLUSIONThe synthesized siRNAs in vitro are capable of increasing the level of cGMP in the smooth muscle cells of human corpus cavernosum, different siRNAs with different capabilities. The siRNA technique could provide not only an extremely powerful tool for the functional analysis of genome but also a new approach to ED gene therapy.
