Establishment of autologous endometrial coculture and sequential system for human early embryo culture.
- Author:
Ning-yuan ZHANG
1
;
Ya-li HU
;
Hai-xiang SUN
;
Bin WANG
;
Zhi-peng XU
;
Hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Coculture Techniques; Culture Media; Embryo, Mammalian; cytology; Endometrium; cytology; Female; Humans
- From: National Journal of Andrology 2006;12(11):997-1003
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a coculture and a sequential system for human early embryo culture.
METHODSThe endometrial tissue was digested enzymatically and cultured to achieve generated and cryo-thawed endometrial monolayer cells. The generated and cryo-thawed monolayer cells were cocultured with human 2PN embryos and transferred to sequential medium every 48 hours.
RESULTSHuman endometrial cells had viability in vitro culture. The autologously generated and cryo-thawed monolayer cells were successfully obtained, and 74.04% of the cryo-thawed cells were successfully used in coculturing human early embryos. The embryos developed well, with the clinical pregnancy rate of 68.83% and the implantation rate of 44.23%.
CONCLUSIONThe autologous endometrial cell coculture and sequential culture system for human early embryo development provides a feasible method for studying human embryo development and implantation so as to improve embryo quality.
