Expression of Bax and Bcl-2 gene in rat testis tissues induced by testicular denervation.
- Author:
Yong-guang GONG
1
;
Yu-ru YANG
;
Wei ZHAGN
;
Min GU
;
Chang-jun YIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Denervation; Leydig Cells; metabolism; Male; Proto-Oncogene Proteins c-bcl-2; biosynthesis; Rats; Rats, Sprague-Dawley; Spermatogonia; metabolism; Testis; innervation; metabolism; bcl-2-Associated X Protein; biosynthesis
- From:National Journal of Andrology 2006;12(12):1063-1065
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the changes of the expression of Bax and Bcl-2 gene in the denervated testis, and to explore the possible mechanisms underlying the apoptosis of germ cells induced by testicular denervation at the genetic translation level.
METHODSEighteen mature SD rats (350-375 g) were equally divided into 3 groups: a sham operation group( SO) , a superior spermatic nerve group (SSN) and an inferior spermatic nerve group (ISN) , and the latter two received bilateral surgical removal of the superior spermatic nerve and the inferior spermatic nerve, respectively. The animals were killed I month after the operation. ISH SP-method was used to detect the expression of Bax and Bcl-2 protein.
RESULTSSignificant up-regulation of Bax protein was detected in both the treatment groups 1 month after surgery( P <0. 05) , and the level of Bcl-2 protein remained unchanged.
CONCLUSIONBax gene is involved in the apoptosis of germ cells induced by testicular denervation.
