Establishment of an animal model of azoospermia in male mice.
- Author:
Xun-bin HUANG
1
;
Hong-gang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Azoospermia; Busulfan; administration & dosage; Cyclophosphamide; administration & dosage; Disease Models, Animal; Estradiol; administration & dosage; Injections, Intraperitoneal; Injections, Subcutaneous; Male; Mice; Mice, Inbred C57BL
- From: National Journal of Andrology 2007;13(2):147-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a stable animal model of azoospermia in male mice.
METHODSTwo groups of mice, with 30 in each, were intervened respectively by chemotherapy (intraperitoneal injection of Busulfan and Cyclophosphamide) and subcutaneous injection of Estradiol. At different times after the injection, the microscopic structures of the seminiferous tubules of both groups were observed and compared. The recovery of the germ cells in the seminiferous tubules was also evaluated after the termination of the intervention.
RESULTSA stable animal model was established by chemotherapeutic method with Busulfan and cyclophosphamide, while the model constructed by muscle injection of Estradiol was not stable.
CONCLUSIONCompared with estrogen injection, chemotherapeutic intervention is a reliable method for constructing an animal model of azoospermia in male mice.
