Immortalized human odontoblast-like cell line expressed dentin extracellular matrix in vitro.
- Author:
Han-guo WANG
1
;
Ming-zhen XIAO
;
Shou-liang ZHAO
;
Jian-jun HAO
;
Min ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Collagen Type I; Dentin; Extracellular Matrix; Extracellular Matrix Proteins; Humans; Immunohistochemistry; In Situ Hybridization; In Vitro Techniques; Odontoblasts; Phosphoproteins; RNA, Messenger; Sialoglycoproteins
- From: West China Journal of Stomatology 2005;23(6):518-521
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro.
METHODSCollagen type I, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP) were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week.
RESULTSDSPP, DMP1, BSP and collagen type I were expressed in hTERT-hOd-1 either at mRNA or protein level. Under the induction of mineralizing medium, the cells showed higher activity of ALP and increased secretion of OC.
CONCLUSIONhTERT-hOd-1 expressed dentin extracellular matrix in vitro, which means the cell line has the potential of mineralization.
