Characteristics of free Ca2+ distribution in cultured osteoclast-like cells.
- Author:
Xiang-jun BAO
1
;
Xing LIANG
;
Ming CHEN
;
Hang WANG
;
Hong-jie SONG
;
Bao-min ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Aniline Compounds; Calcium; Cell Line; Cells, Cultured; Microscopy, Confocal; Osteoclasts; Xanthenes
- From: West China Journal of Stomatology 2006;24(1):18-20
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the spatial distribution of free Ca2+ in osteoclast-like cells cultured on glass.
METHODSTo detect the free Ca2+ in osteoclast-like cells, the images were analyzed with image software, using the laser scanning confocal microscope and fluorescent probe.
RESULTSAt 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM, the intensity of Ca2+ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly.
CONCLUSIONThe intensity of Ca2+ fluorescent signal is different among various organellae in osteoclast-like cell, which suggests the osteoclast-like cell modulate its own function through the spatial difference of free Ca2+ concentration.
