Transcriptional regulation of dentin sialophosphoprotein by c-Jun/c-Fos.
- Author:
Wen-xi HE
1
;
Zhong-ying NIU
;
Shou-liang ZHAO
;
Ping LI
;
Jie GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Extracellular Matrix Proteins; Gene Expression Regulation; Mice; Odontoblasts; Phosphoproteins; Promoter Regions, Genetic; Sialoglycoproteins; Transfection
- From: West China Journal of Stomatology 2006;24(1):67-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein (DSPP) gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23.
METHODSEndogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene.
RESULTSc-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter.
CONCLUSIONThese findings suggest c-Jun and c-Fos down-regulated the transcription of DSPP gene as a transcriptional factor in odontoblast.
