Effect on apoptosis of Tca8113 of interference of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides transfection.

Ping JI; Yong-zhong WU; Fu-jun Zhang; Shao-lin Lin

Return

Effect on apoptosis of Tca8113 of interference of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides transfection.

Author: Ping JI ¹ ; Yong-zhong WU ; Fu-jun ZHANG ; Shao-lin LIN
Author Information

1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China.
Publication Type:Journal Article
MeSH: Apoptosis; Female; Genes, erbB-2; Humans; Oligodeoxyribonucleotides; Oligodeoxyribonucleotides, Antisense; Transfection
From: West China Journal of Stomatology 2006;24(2):156-159
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the interference and the mechanisms of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection in the human tongue carcinoma Tca8113 cell lines.
METHODSThere were 6 groups in our study, normal control group, Bcl-2 sense experimental group, HER-2 sense experimental group, Bcl-2 antisense experimental group, HER-2 antisense experimental group, Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection experimental group. In the different times after liposome-mediated transfection, the cell apoptosis, Bcl-2 and HER-2 expressing level were observed by RT-PCR and electronic microscope.
RESULTSAccording to the results of combined transfection experimental group, the apoptosis body and apoptosis cells were observed. The expression of genes were decreased statistically in both Bcl-2 and HER-2, respectively. Bcl-2 and HER-2 combined ASODN was superior to single ASODN in the intervention of tongue carcinoma.
CONCLUSIONBcl-2 and HER-2 ASODN can effectively interfere the expression of HER-2 and Bcl-2 genes. The expression of HER-2 and Bcl-2 can be reduced, and the apoptosis of cells can be enhanced.