Quantitative study of osteoclastic related factors in the process of bone reconstruction.

Author: Xin CHANG ¹ ; Zhi-ming HOU ; Yasuaki SIBATA ; Tomoo TSUKAZAKI ; Akira YAMAGUCHI
Author Information

1. Dept. of Oral Science, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
Publication Type:Journal Article
MeSH: Animals; Bone Resorption; Male; Mice; Mice, Inbred C57BL; NF-kappa B; Osteoclasts; Osteogenesis; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Tumor Necrosis Factor-alpha
From: West China Journal of Stomatology 2006;24(2):164-169
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling.
METHODS8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR.
RESULTSTNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury.
CONCLUSIONOsteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.