Effect on xenograft of nude mouse by combination therapy of nm23-H1 and protein-cisplatin.

Author: Ke-qian ZHI ¹ ; Wei-hui CHEN ; Yu-ming WEN
Author Information

1. Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.
Publication Type:Journal Article
MeSH: Animals; Cisplatin; Female; Heterografts; Mice; Mice, Inbred BALB C; Mice, Nude; NM23 Nucleoside Diphosphate Kinases; Neoplasm Transplantation; Transfection
From: West China Journal of Stomatology 2006;24(2):170-172
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the effect of protein-cisplatin and nm23-H1 therapy on the tumor of nude mouse.
METHODSThe 15 BALB/C female mice were divided into three groups, control group, protein-cisplatin group and protein-cisplatin+nm23-H1 plasmid group. Tca8113 were injected into the mice subcutaneously with the concentration of 3.1 x 10(6) cells/mL. After two weeks, the mixture of lipofectin and nm23-H1 was injected around xenograft of nude mouse. After three days, the protein-cisplatin was injected around xenograft. The weight of mouse, the volume and the weight of xenograft were measured.
RESULTSThe weight of mouse was lightest in control group. The volume and weight of the transplanted tumor were lightest in nm23-H1 +protein-cisplatin group.
CONCLUSIONThe combination therapy of nm23-H1 and protein-cisplatin can effectively inhibites the growth of xenograft in nude mouse.