Experimental studies of human adipose tissue-derived stromal cells transfected with ad-hBMP-2 gene.
- Author:
Pei-hui ZHENG
1
;
Feng-cai WEI
;
Guo-ying JIN
;
Xiu-li SHAN
;
Shu-yang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; Adipocytes; Adipose Tissue; Animals; Bone Morphogenetic Protein 2; Cells, Cultured; Genetic Vectors; Humans; Rats; Rats, Sprague-Dawley; Stromal Cells; Tissue Engineering; Transfection
- From: West China Journal of Stomatology 2006;24(3):195-198
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possibility of adipose-derived stromal cells (ADSCs) transfeced by adenovirus containing human bone morphogenetic protein-2 (Ad-hBMP-2) gene and their osteogenic potential.
METHODSADSCs were obtained from inguinal fat tissue of 4 weeks old SD rats. After exposure to adenovirus containing green fluorescent protein(Ad-GFP), fluorescent microscope was used to observe gene transfection effect once 12 hours. After transfected with Ad-hBMP-2, cytochemistry, immmucytochemistry and Western blot were used to examine the expression of alkaline phosphatase (ALP), osteocalcin (OC) and hBMP-2.
RESULTSAfter exposed to Ad-GFP 12 hours, 52% ADSCs were observed being transfected and 48 hours later reached 95%. The double number time belonged after transfecting with Ad-hBMP-2, and cytochemistry, immucytochemistry and Western blot examines indicated positive results of ALP, OC, hBMP-2 after 48 hours.
CONCLUSIONAdipose tissue contains abundant ADSCs which could be transfected as gene vectors by adenovirus, ADSCs transfected with Ad-hBMP-2 can convert to ostoeblasts, and can act as a kind of seed cells for osteo-tissue engineering.
