Effects of overdosed fluoride on rat's incisor expression of matrixmetalloproteinase-20 and tissue inhibitors of metalloproteinase-2.
- Author:
Feng-qiu JING
1
;
Qiang WANG
;
Tian-lin LIU
;
Li-ying GUO
;
Hui LIU
Author Information
- Publication Type:Journal Article
- MeSH: Ameloblasts; Animals; Dental Enamel; Fluorides; Fluorosis, Dental; Incisor; Metalloproteases; Phosphates; Rats; Rats, Wistar; Tissue Inhibitor of Metalloproteinase-2
- From: West China Journal of Stomatology 2006;24(3):199-201
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of overdosed fluoride on the expression of MMP-20 and TIMP-2 in rat incisor.
METHODS20 Wistar rats were randomly divided into experiment group and control group. The distilled water was given in control group. 100 mg/L fluoride- was given in experiment group. After 8 weeks treatment, the rats were killed. Immunohistochemical staining was used to observe the expression of MMP-20 and TIMP-2 in rat incisors.
RESULTSImmunohistochemical results demonstrated the presence of MMP-20 and TIMP-2 protein in ameloblasts, odontoblasts, stratum intermedium and the stellate reticulum of rat incisor. The imagination analysis results showed that the expression of MMP-20 was reduesed in experiment group (P<0.01), and the expression of TIMP-2 had no significant difference (P>0.05).
CONCLUSIONThe overdosed fluoride inhibits the secretion of MMP-20 and leads to the disturbed balance between MMP-20, TIMP-2 in rat incisor, which leads to the delay of the amelogenin removal and the enamel demineralization.
