Regulation of PTHrP in proliferation and differentiation of chondrocytes of condyle in fetal mouse.
- Author:
Lai-kui LIU
1
;
Hong-bing JIANG
;
Xin-min YIN
;
Xiao-qing LI
;
Xin-zhu YI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Chondrocytes; Growth Plate; Humans; Mice; Parathyroid Hormone-Related Protein
- From: West China Journal of Stomatology 2006;24(3):206-209
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory mechanism of parathyroid hormone-related protein (PTHrP) in proliferation and differentiation of chondrocytes of condyle in fetal mouse.
METHODSChondrocytes of condyle in fetal mouse were separated and cultured in vitro, the influence of PTHrP on proliferation and differentiation was observed.
RESULTSAfter two weeks' culture in 0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10 nmol/L human PTHrP, there was significant difference in the number of cartilage nodule formed between experiment group and control group (P<0.05), and there was no significant difference in 0.01 nmol/L group (P>0.05). Alkaline phosphatase (ALP) activity was significantly intensified in experiment group and control group (P<0.05). Meanwhile, it was found that this function of promotion was lessened after anti-PTHR antibody used.
CONCLUSIONIt can be seen that PTHrP, via its receptor, can promote proliferation and differentiation of chondrocytes of condyle, which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.
