Effect of overdose fluoride on the expression of enamelin in rat mandibular incisor.
- Author:
Tian-lin LIU
1
;
Qiang WANG
;
Zhi-ling XU
;
Li-ying GUO
;
Feng-qiu JING
;
Gang WU
Author Information
- Publication Type:Journal Article
- MeSH: Ameloblasts; Animals; Dental Enamel; Dental Enamel Proteins; Fluorides; Incisor; Mandible; Odontoblasts; Phosphates; Rats; Rats, Wistar
- From: West China Journal of Stomatology 2006;24(4):297-299
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of overdose fluoride on the expression of enamelin in rat mandibular incisor.
METHODSTwenty Wistar rats were divided randomly into two groups. Animals were maintained in standard environment with free access to food and distilled water (control group) or water added with 100 mg/L F-(experimental group). The rats were killed in the eighth week. HE staining was used to observe the morphology of ameloblasts. Immunohistochemical staining was adopted to study the expressions of enamelin in rat incisor.
RESULTSThe ameloblasts of the treated rat were arranged in multi-layer. The ameloblasts in group II were thinner than those in group I. The structure of enamel matrix was in disorder. The expressions of enamelin in ameloblasts and odontoblasts were obviously inhibited in group II (P < 0.01).
CONCLUSIONThe overdose fluoride inhibits the secretion of enamelin and leads to the abnormal development of enamel matrix.
