Experimental studies on ectopic osteogenesis by seeding human mesenchymal stem cell on human natural bone derived scaffold materials.
- Author:
Fei-wu KANG
1
;
Xiu-fa TANG
;
Yu-ming WEN
;
Zheng-hua WU
;
Xin HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone and Bones; Cells, Cultured; Humans; Mesenchymal Stromal Cells; Mice; Mice, Nude; Osteogenesis; Tissue Engineering
- From: West China Journal of Stomatology 2006;24(4):357-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the ectopic osteogenesis potential of human natural bone derived material combined with human bone marrow mesenchymal stem cells (MSCs).
METHODSCell-scaffold complexes were implanted subcutaneously into the left back of the nude mice, and human natural bone derived material were implanted into the right back as control group. The mice were killed respectively on the postoperative 2 weeks, 4 weeks and 8 weeks. The macroscopic, histopathological, alkaline phosphatase (ALP) activity assay methods were performed to assess the ectopic osteogenesis potential.
RESULTSThe cartilaginous osteogenesis were observed in both deproteinated bone and decalcified bone, and the more new bone tissue formed gradually as the time went by after implantation. ALP activity become stronger followed with the time (P < 0.05), and compared with the decalcified bone, deproteinated bone displayed stronger ALP activity (P < 0.05).
CONCLUSIONThe MSCs and human natural bone derived material can be used as good seed cells and scaffold materials respectively to construct tissue-engineered bone, and as the scaffold material, deproteinated bone has better osteogenesis ability than decalcifed bone.
